Zeiss LSM 510 Meta (Room A503b)

Laser scanning confocal microscope for both fixed and live cell imaging. Mounted on a motorized Zeiss Axiovert 200M inverted microscope. Spectral Meta detector for emission fingerprinting. Bleaching routines for FRET and FRAP.

Equipped with Zeiss Heating Insert P, Tempcontrol 37-2 digital and Zeiss objective heater.

Leica TCS SP2 confocal (Room A123b)

Laser scanning confocal microscope with a full-enclosure type temperature-controlled environmental chamber for live-cell imaging and bleaching (FRAP) applications. Fully motorized DM RXA2 upright microscope. Available laser excitation lines 458, 476, 488, 496, 514, 561 and 633 nm, spectral detectors for four fluorescent channels and one for transmitted light. Reflectance mode for material surface analyses.

Zeiss LSM 5 Duo (Room A503b)

Laser scanning confocal microscope with two scan heads: LSM 510 point-scanner, and LSM 5 Live line-scanner that has maximum speed of 120 frames/s with 512x512 pixels. Microscope inverted Zeiss Axio Observer Z1. Large incubator XL-LSM S1 with temperature, CO₂, and humidity control. Motorized stage with piezo focus.

Duo is suitable both for fixed and live cell imaging. Live scan head enables very fast confocal microscopy. Bleaching for freely selectable area using the point-scanner, together with fast line-scanning, makes Duo ideal for FRAP, FRET, and other photobleaching/activation applications.

Zeiss / Intelligent Imaging Innovations (3i) - Stallion HSI (Room B501b)

Time-lapse live cell imaging system for high-speed, multichannel 4D (Z-stacks over time) imaging. Motorized Zeiss 200M inverted microscope with an environmental chamber (temperature and CO2 control). Autofocus, montage, and multipoint acquisition possible.

TILL Photonics wide-field (Room A123b)

Fast inverted live-cell imaging system with multi-wavelength excitation range from UV to near IR. Equipped with a full-enclosure type temperature-controlled environmental chamber for prolonged image acquisition protocols.

Nikon Eclipse Ti-E N-STORM imaging system (Room B115a2)

Nikon Eclipse Ti-E inverted widefield microscope with a 4 laser illumination unit for STORM super-resolution and TIRF imaging. The system has 405, Argon, 561 and 647 laserlines and is equiped with a Nikon PFS (Perfect focus system) for live cell imaging.

Caliper IVIS 100 Imaging System (Haartman institute)

Bioluminescense and fluorescence imaging of live animals. The imaging chamber has room for up to five mice or two rats. Peltier cooled, back-thinned, back-illuminated CCD camera for image acquisition and Living Image 2.20 software. Equipped with a gas anesthesia system.

Caliper IVIS Kinetic imaging system (Room AP27a2)

Caliper IVIS Kinetic optical small animal in vivo imaging system and Xenogen XGI-8 anesthesia system Andor iXon+ 888 EMCCD camera enables fast kinetic imaging of biological functions. Equipped with a standard high spectral resolution filter set (excitation 430-745 nm and emission 515-875 nm) and a 500 series high spectral resolution emission set (500-620 nm) for spectral unmixing. Imaging modalities include fluorescence, luminescence and white light. Living Image 3.2 software for aquisition and analysis.

Magnetic Resonance Imaging (MRI)

A 4.7 Tesla basic versatile small animal MRI equipped with strong gradients allowing for quick imaging of virtually all organs. For enquiries please follow the link: www.hfic.helsinki.fi/mri/

Optical Projection Tomography (OPT) (Left of Room A513)

The Bioptonics OPT 3001M Scanner is an optical projection tomography platform that produces high-resolution (15-30 microns) 3-Dimensional image reconstructions of both fluorescently stained and non-fluorescent specimens ranging in size from 1mm to 15mm. Applications include visualization of anatomy (phenotyping), gene expression (in situ hybridization), protein distribution (immunohistochemistry or GFP expression), transgenic visualization (LacZ). Typical specimens include mouse and chicken embryos, mouse/rat tissue and organs, zebrafish (5 days and older), drosophila, plants etc.

Thermo Scientific CellInsight (Room B501b)

The old Cellomics ArrayScan 4.5 has been replaced with a new Thermo Scientific CellInsight system. The new system is fully compatible with the ArrayScan.

Cell-based high-content screening system for automated fluorescence image aqcuisition and quantitative analysis. Ideal for both RNAi and chemical compound screens. Up to four different fluorochromes can be used.

With CellInsight, you can study e.g. biochemical targets, signaling pathways, organelle status, phenotypic changes, and cell functions. Special bioapplications are available, for example cell cycle, motility, spreading, and viability analysis, as well as for molecular translocation, GPCR signaling, neurite outgrowth, and cytotoxicity.

Imaging workstation 1 (Room B501a lobby)

Dell Precision 530 MT with Dual Xeon 3.2 GHz CPU, 4 GB RAM and dual monitors. Workstation for image processing and analysis (also in 3D and 4D): ImageProPlus for image processing and analysis; Autoquant X AutoDeblur for blind deconvolution; Zeiss LSM software for confocal images; SlideBook for editing time-lapse imaging data (e.g. movie editing); Living Image for analysis of IVIS data and other imaging software. HP G4050 scanner for paper, film, and gels. Brady barcode printer for making barcodes.

Imaging workstation 2 (Room B501a lobby)

Osborne with Intel Core2 Duo 3.0 GHz CPU, 4 GB RAM and dual monitors. Workstation for disk-based (virtual) rescanning and analysis of high-content screening data using Cellomics DiscoveryToolbox software. Spotfire DecisionSite 3D data visualization software; ImageProPlus for image processing and analysis; Living Image for analysis of IVIS data. IgorPro for graphing as well as data and image analysis. Also other imaging software.

Imaging workstation 3 (Room A125b)

HP xw9400 with quadruple AMD Opteron 64-bit 3.0 GHz CPU, 16 GB RAM, dual monitors. SVI Huygens Professional deconvolution with confocal, wide-field and time modules. Bitplane Imaris volume rendering software with measurement and co-localization modules. Readers available for most image file formats.

Microscope 1: Zeiss Axioplan 2 (Room B501b)

Upright microscope for transmitted light bright-field, phase contrast and DIC microscopy of fixed specimen. Capable of limited epifluorescent imaging. Image acquisition using Zeiss digital AxioCam RGB color camera and AxioVision software.

Microscope 2: Zeiss Axioplan 2 (Room B501b)

Upright microscope epifluorescence microscopy of fixed specimen. Limited transmitted light imaging capability. Image acquisition using Zeiss digital AxioCam grayscale camera and AxioVision software.

Leica DM LB (Leica)

Research microscope for bright-field microscopy. Image acquisition through Olympus digital camera and Studio Lite software.