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Biomedicum Sequencing Unit (BSU)

Contact Information:

Tapio Tainola

Biomedicum Helsinki
Room A510
PO Box 63 (Haartmaninkatu 8)
FI-00014 University of Helsinki
Finland

Tel. +358 9 191 25565
Fax. +358 9 191 25510

e-mail :
firstname.surname@helsinki.fi

Details and prices

BSU sequences customer's DNA templates (plasmid DNA, PCR products) by using Applied Biosystems Big Dye Terminator (v1.1) kit for PCR and Applied Biosystems ABI Prism 3130xl 16 capillary Genetic Analyzer for analyzing. The Facility offers M13-F, M13-R, T7, T3 and SP6 primers, if special primers are required they should be provided by the customer.

Prices:

 
Price / €
(Excl. VAT)
Price / €
Incl. VAT 22 %
  • Normal prices per template, primer, sequencing-PCR, purification and analyzing
13,93
17,00
  • Analysis only (sequencing PCR-reaction is prepared by customer).
5,74
7,00
  • Full 96 well plate. (sequencing PCR-reaction is prepared by customer)
394 ,00
480,00
     
Required amounts of templates:
Plasmid-DNA 500 ng - 1 µg (100 ng/1kb)
PCR-products:
  • 100-200 bp 5 ng
 
  • 200-500 bp 10 ng
 
  • 500 -1000 bp 20 ng
 
  • 2 kb-3kb 50 ng
  3.2 pmol primer per reaction
Add deionized water to bring total volume of 12 µl, When customer wants to use the primers provided, the total volume should be 11 µl.

Results will be available at least earliest the next morning, if samples are brought before 9 am.

Page updated October 11, 2005

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