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Faculty of Medicine
Biomedicum Helsinki
 
Biomedicum Äkta Explorer FPLC Core Facilty: Equipment

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Contact Information:

Michael Jeltsch

Biomedicum Helsinki
Room A510
PO Box 63 (Haartmaninkatu 8)
FI-00014 University of Helsinki
Finland

Tel. +358 9 191 25514
Fax. +358 9 191 25510

MICHAEL@JELTSCH.ORG
http://jeltsch.org
skype jeltsch

Prepacked columns. We have a modest choice of prepacked columns. Since they are not cleaned after every use, their condition (especially that of the rarely used columns) is sometimes unknown. They might require cleaning/sanitation before use.

Single-use columns. A good alternative for many applications are single use columns, which you need to provide yourself. We have a small stock of some of the more frequently used types which you can borrow if you are in a hurry.

Empty columns.The empty columns are vailable for packing, but occassionally one or the other might be packed with expesive media for multiple use by some user and is therefore occupied for longer periods of time.

Resins/media. Some of the bulk media is available for free as the smallest available package sizes are so large that the media anyway expires before anybody can use it to the end.

Prepacked columns

  • Ion exchange
    • 1x Mono Q HR 5/5
    • 1x Mono Q 5/50 GL
    • 1x Resource Q 1ml
    • 1x Mono S HR 5/5
    • 1x Mono S 5/50 GL
    • 1x Mini S 4.6/50 PE
    • 1x Resource S 1ml
  • Gel filtration/size exclusion
    • 1x HR 10/10 fast desalting column
    • 1x HR 10/30 Superdex 200
    • 2x Superdex 75 10/300 GL
    • 1x Superdex 200 10/300 GL
    • 1x HiLoad 26/60 Superdex 200 pg
    • 1x HiLoad 26/60 Superdex 75 pg
  • Reverse phase
    • PepRPC HR 5/5

Single-use columns

  • Ion exchange
    • 1ml and 5ml HiTrap Q HP
    • 1ml HiTrap S HP
  • Affinity
    • 1ml and 5ml Heparin HP
  • Metal chelate
    • 1ml HisTrap HP

Empty columns

  • Tricorn 5mm
    • 2x 5/50 columns with standard adaptor units
    • 1x 5/150 glass tube
    • 1x packing connector (to make one 100mm column out of two 50mm colums or for column filling)
  • Tricorn 10mm
    • 1x 10/50 glass tube
    • 2x adapter unit
  • HR 5mm
    • 2x 5/50 columns
  • HR 10mm
    • 1x 10/10 column
  • XK16
    • 2x XK16/20 columns including column packing kit

Resins/media

  • Ion exchange
    • SP Sepharose Fast Flow
    • DEAE Sepharose CL-6B
  • Gel filtration/size exclusion
    • Sephadex G-100, G-50, G-15
    • Sephacryl S-500 HighRes
    • Sepharose 4 FastFlow
  • Metal chelate
    • Ni2+ NTA Superflow
    • Ni2+ NTA Agarose
    • Chelating Sepharose Fast Flow
  • Affinity
    • Heparin Sepharose 6 Fast Flow
    • IgG Sepharose 6 Fast Flow
    • ConA Sepharose
  • Other
    • CNBr-activated Sepharose 4B
    • Ceramic Hydroxyapatite

Page updated January 25, 2011

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