Protein Production and Purification (Äkta Avant FPLC & WAVE Bioreactor)

 

Protein Production and Purification Core Facility
(Äkta Avant FPLC & WAVE Bioreactor)
Faculty of Medicine
Biomedicum Helsinki
 

    Michael Jeltsch
    Ph.D., Adjunct Professor, Academy Research Fellow

    Translational Cancer Biology Research Program
    Institute of Biomedicine
    Biomedicum Helsinki

    P.O. Box 63 (Haartmaninkatu 8)
    FI-00014 University of Helsinki
    Finland

    Tel. +358 2 941 25514
    Fax +358 2 941 25510

    michael@jeltsch.org
    firstname.surname@helsinki.fi

Affiliations

Alma-intranet Flammaintra

Large equipment

Äkta Avant

Äkta Explorer (will be discontinued soon)

WAVE Bioreactor 20/50EHT

Savant SpeedVac SPD121P

Incubators/Shakers

Columns and resins

Prepacked columns

We have a modest choice of prepacked columns. Since they are not cleaned after every use, their condition (especially that of the rarely used columns) is sometimes unknown. They might require cleaning/sanitation before use.

Ion exchange
  • 1x Mono Q HR 5/5
  • 1x Mono Q 5/50 GL
  • 1x Resource Q 1ml
  • 1x Mono S HR 5/5
  • 1x Mono S 5/50 GL
  • 1x Mini S 4.6/50 PE
  • 1x Resource S 1ml
Reverse phase
  • PepRPC HR 5/5

Single-use columns

A good alternative for many applications are single use columns, which you need to provide yourself. We have a small stock of some of the more frequently used types which you can borrow if you are in a hurry.

Ion exchange
  • 1ml and 5ml HiTrap Q HP
  • 1ml HiTrap S HP
Affinity
  • 1ml and 5ml HiTrap Q HP
  • 1ml HiTrap S HP
Metal chelate
  • 1ml HisTrap HP

Empty columns

The empty columns are vailable for packing, but occassionally one or the other might be packed with expesive media for multiple use by some user and is therefore occupied for longer periods of time.

Tricorn 5mm
  • 2x 5/50 columns with standard adaptor units
  • 1x 5/150 glass tube
  • 1x packing connector (to make one 100mm column out of two 50mm colums or for column filling)
Tricorn 10mm
  • 1x 10/50 glass tube
  • 2x adapter unit
HR 5mm
  • 2x 5/50 columns
HR 10mm
  • 1x 10/10 column
XK 16mm
  • 2x XK16/20 columns including column packing kit

Resins/media

Some of the bulk media is available for free as the smallest available package sizes are so large that the media anyway expires before anybody can use it to the end.

Ion exchange
  • SP Sepharose Fast Flow
  • DEAE Sepharose CL-6B
Gel filtration/size exclusion
  • Sephadex G-100, G-50, G-15
  • Sephacryl S-500 HighRes
  • Sepharose 4 FastFlow
Metal chelate
  • Ni2+ NTA Superflow
  • Ni2+ NTA Agarose
  • Chelating Sepharose Fast Flow
Affinity
  • Heparin Sepharose 6 Fast Flow
  • IgG Sepharose 6 Fast Flow
  • ConA Sepharose
Other
  • CNBr-activated Sepharose 4B
  • Ceramic Hydroxyapatite

Enzyme lists

We still use fairly often old-fashioned restriction enzyme cloning to assemble our expression constructs. If you only need a few microlitres (or if you are waiting for your enzyme to arrive and want to borrow something for the initial fragment generation), please ask!
* Displaying our enzyme list works only inside the Helsinki University network since it polls our intranet server directly!

Restriction enzymes*

Other enzymes*