Michael Jeltsch
Ph.D., Adjunct Professor, Academy Research Fellow
Translational Cancer Biology Research Program Institute of Biomedicine Biomedicum Helsinki
P.O. Box 63 (Haartmaninkatu 8) FI-00014 University of Helsinki Finland
Tel. +358 2 941 25514 Fax +358 2 941 25510
michael@jeltsch.org firstname.surname@helsinki.fi
Affiliations
From the beginning of 2016, the facility will run as an official core facility of the medical faculty. Every new user needs to be registered and receive a minimum of 1 hour training. Training, use and purified proteins are free for all collaborative work.
| Internal academic customers (Helsinki University (HU) & HU Hospital | External academic customers | Non-academic customers |
| Equipment use* | |||
| Äkta Avant | 32€/run | 48€/run | 96€/run |
| WAVE reactor | 142€/run | 213€/run | 426€/run |
| Incubator space | 0.26€/flask/d | 0.39€/flask/d | 0.78€/flask/d |
| *This does not include buffers and columns. Disposable columsn are provided by the customer. Maximum length of a run is 24 hours for the Äkta Avant and 7 days for the WAVE reactor. Longer run times are charged proportionally. | |||
| Training and assisted use | |||
| Training* | 24€/h | 36€/h | 72€/h |
| Assisted use | 24€/d | 36€/d | 72€/d |
| Course** | 2000€ | 3000€ | 6000€ |
| * Training sessions are usually 1.5 hours. Pricing is independet of the amount of participants, but the maximal anumber is limited to four. E.g. 1.5 hours of training cost 32€ (Äkta run) + 1.5x24€ (training) = 68€ for an internal academic customer. ** Five-day course (~4h training/day). We purify a protein via three columns (affinity, IEX/HIC/etc., gel filtration) and analyze the results via PAGE + Western/Coomassie/Silver staining. Maximal number of participants: 8. We are purifying one protein for each group of 4 participants. | |||
| Columns | |||
| gel filtration 30cm, fast desalting | 8€/d | 12€/d | 24€/d |
| gel filtration 60cm | 12€/d | 18€/d | 36€/d |
| IEX an others | 20€/d | 30€/d | 60€/d |
| Column and/or machine cleanup* | 24€/h | 36€/h | 72€/h |
| * Columns are provided in the appropriate storage buffer (e.g. 20% ethanol for gel filtration columns). After use, they need to be returned clean and in the same buffer. Users are also expected to flush the tubing of the machine after use in order to prevent salt depositions or microbial growth inside the device. If we have to clean the device or clean or buffer exchange rented/borrowed columns because the user failed to do so, we will ask for a fee as this takes quite some time and effort. Cleaning by the user does not include the cleaning-in-place (CIP) with (e.g. 0.5 M NaOH for gel filtration columns), which we do regularily every 10 runs. | |||
| Purified proteins (standardized production service*) | |||||||||||
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| * This service is provided for proteins, for which we have performed the production and purification many times and for which the production and purification procedure has been published. We perform extensive purity and activity assays on these proteins before we hand them over to our customers. | |||||||||||
| Purified proteins (customized production service) | |||
| We are offering the production of recombinant proteins as a full service (starting from cloning of the expression construct to the final purified protein). We offer two standard expression
systems with in-house developed vectors: stably transfected insect cells (Drosophila S2) and stably transfected mammalian cells (CHO). Our standard purification consists of two-steps: affinity purification (via a polyhistidine tag or and antibody Fc tag) and gel filtration. However, we recognize the need of non-standard procedures for many proteins and therefore we offer also customized solutions (different vectors, different expression hosts, different purification schemes). Please contact us for details and pricing! |
Reservations for the equipment are done online. Everybody within the Helsinki University network can view the reservations, but in order to make a reservation, you need to login.